Ligustrum vulgare leaves and fruit extracts induce apoptosis of human leukemia cells

Background and purpose: Cytotoxic effects of Ligustrum vulgare leaves on HeLa cervical tumor cells suggested that extracts should be investigated as potential anticancer agents. Therefore, we examined a antileukemic activity methanolic fruit two types leukemia cells, MOLT-4 JVM-13, lymphocytes isolated from the blood 33 chronic lymphocytic (CLL) patients mononuclear leukocytes 18 healthy individuals.Material methods: The cytotoxicity was measured by MTT assay LDH test. antiapoptotic tested Annexin V/7AAD flowcytometric assay.Results: results showed both exhibited moderate cytotoxic effect all three cells. leaf extract most effective JVM-13 were equally CLL In addition, none toxic to Both acted inducing apoptosis leukemic cells.Conclusion: exhibit significant further investigated.